Protection against a lethal H5N1 influenza challenge by intranasal immunization with virus-like particles containing 2009 pandemic H1N1 neuraminidase in mice
Identifieur interne : 000A24 ( Main/Exploration ); précédent : 000A23; suivant : 000A25Protection against a lethal H5N1 influenza challenge by intranasal immunization with virus-like particles containing 2009 pandemic H1N1 neuraminidase in mice
Auteurs : Judith D. Easterbrook [États-Unis] ; Louis M. Schwartzman [États-Unis] ; Jin Gao [États-Unis] ; John C. Kash [États-Unis] ; David M. Morens [États-Unis] ; Laura Couzens [États-Unis] ; Hongquan Wan [États-Unis] ; Maryna C. Eichelberger [États-Unis] ; Jeffery K. Taubenberger [États-Unis]Source :
- Virology [ 0042-6822 ] ; 2012.
Abstract
Highly pathogenic H5N1 influenza shares the same neuraminidase (NA) subtype with the 2009 pandemic (H1N1pdm09), and cross-reactive NA immunity might protect against or mitigate lethal H5N1 infection. In this study, mice were either infected with a sublethal dose of H1N1pdm09 or were vaccinated and boosted with virus-like particles (VLP) consisting of the NA and matrix proteins, standardized by NA activity and administered intranasally, and were then challenged with a lethal dose of HPAI H5N1 virus. Mice previously infected with H1N1pdm09 survived H5N1 challenge with no detectable virus or respiratory tract pathology on day 4. Mice immunized with H5N1 or H1N1pdm09 NA VLPs were also fully protected from death, with a 100-fold and 10-fold reduction in infectious virus, respectively, and reduced pathology in the lungs. Human influenza vaccines that elicit not only HA, but also NA immunity may provide enhanced protection against the emergence of seasonal and pandemic viruses.
Url:
DOI: 10.1016/j.virol.2012.06.003
PubMed: 22727831
PubMed Central: 3725556
Affiliations:
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Morens</name><affiliation wicri:level="2"><nlm:aff id="A3">Office of the Director, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Office of the Director, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Couzens, Laura" sort="Couzens, Laura" uniqKey="Couzens L" first="Laura" last="Couzens">Laura Couzens</name><affiliation wicri:level="2"><nlm:aff id="A2">Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Wan, Hongquan" sort="Wan, Hongquan" uniqKey="Wan H" first="Hongquan" last="Wan">Hongquan Wan</name><affiliation wicri:level="2"><nlm:aff id="A2">Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Eichelberger, Maryna C" sort="Eichelberger, Maryna C" uniqKey="Eichelberger M" first="Maryna C." last="Eichelberger">Maryna C. Eichelberger</name><affiliation wicri:level="2"><nlm:aff id="A2">Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Taubenberger, Jeffery K" sort="Taubenberger, Jeffery K" uniqKey="Taubenberger J" first="Jeffery K." last="Taubenberger">Jeffery K. Taubenberger</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Viral Pathogenesis and Evolution Section, Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><idno type="eISSN">1096-0341</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p id="P1">Highly pathogenic H5N1 influenza shares the same neuraminidase (NA) subtype with the 2009 pandemic (H1N1pdm09), and cross-reactive NA immunity might protect against or mitigate lethal H5N1 infection. In this study, mice were either infected with a sublethal dose of H1N1pdm09 or were vaccinated and boosted with virus-like particles (VLP) consisting of the NA and matrix proteins, standardized by NA activity and administered intranasally, and were then challenged with a lethal dose of HPAI H5N1 virus. Mice previously infected with H1N1pdm09 survived H5N1 challenge with no detectable virus or respiratory tract pathology on day 4. Mice immunized with H5N1 or H1N1pdm09 NA VLPs were also fully protected from death, with a 100-fold and 10-fold reduction in infectious virus, respectively, and reduced pathology in the lungs. Human influenza vaccines that elicit not only HA, but also NA immunity may provide enhanced protection against the emergence of seasonal and pandemic viruses.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Easterbrook, Judith D" sort="Easterbrook, Judith D" uniqKey="Easterbrook J" first="Judith D." last="Easterbrook">Judith D. Easterbrook</name></region><name sortKey="Couzens, Laura" sort="Couzens, Laura" uniqKey="Couzens L" first="Laura" last="Couzens">Laura Couzens</name><name sortKey="Eichelberger, Maryna C" sort="Eichelberger, Maryna C" uniqKey="Eichelberger M" first="Maryna C." last="Eichelberger">Maryna C. Eichelberger</name><name sortKey="Gao, Jin" sort="Gao, Jin" uniqKey="Gao J" first="Jin" last="Gao">Jin Gao</name><name sortKey="Kash, John C" sort="Kash, John C" uniqKey="Kash J" first="John C." last="Kash">John C. Kash</name><name sortKey="Morens, David M" sort="Morens, David M" uniqKey="Morens D" first="David M." last="Morens">David M. Morens</name><name sortKey="Schwartzman, Louis M" sort="Schwartzman, Louis M" uniqKey="Schwartzman L" first="Louis M." last="Schwartzman">Louis M. Schwartzman</name><name sortKey="Taubenberger, Jeffery K" sort="Taubenberger, Jeffery K" uniqKey="Taubenberger J" first="Jeffery K." last="Taubenberger">Jeffery K. Taubenberger</name><name sortKey="Wan, Hongquan" sort="Wan, Hongquan" uniqKey="Wan H" first="Hongquan" last="Wan">Hongquan Wan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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